Contact an Account ManagerAnalysis of sulthiame in serum by narrow bore HPLC
Summary
Type: HPLC
Application #: 103860
Catalog#: 720014.30 - MN HPLC Column, Nucleosil C18, 100A, 5um, 3x250mm
Detection: UV 245nm
Conditions: eluent A (= buffer): 0.01% phosphoric acid adjusted to pH 4.5 with 10% aqueous triethylamine
eluent B: buffer - acetonitrile (90:10)
eluent C: buffer - acetonitrile (40:60)
Gradient: 2min 100% A, 2min 60% B, 40% C, gradient to 20% B, 80% C in 5min, the hold at 60% B, 40% A
flow rate 0.6ml/min
temperature 45°C
Authors: Juergens, U.H.
Source: J. Chromatogr. 553 (1991) 7–13
Details
Legend: Chromatogram from the direct injection of a serum containing Ospolot and other
antiepileptic drugs,
ethosuximide
sulthiame (4.7µg/ml)
phenobarbital
carbamazepine epoxide
5-ethyl-5-(p-tolyl) ba